The U-4B was used by President Dwight D. Eisenhower from 1956 to 1960, for short trips. It was the smallest "Air Force One," and the first Presidential aircraft to have only two engines. After Eisenhower left office, the aircraft was used for transporting senior Government officials. On 1 October 1969 it was transferred to the Air Force Academy, where it was used for cadet parachute training and the Academy's skydiving team. In November 1977 the U-4B was sent to the Nebraska Civil Air Patrol. It was obtained by the NMUSAF from a private owner in 1996.

This U-4B was used by President Dwight Eisenhower from 1956 - 1960 for flights from Washington, D.C. to his farm in Gettysburg, VA. From 1961 - 1969 it was used by high ranking government officials for shorter flights. Transferred to the USAF Academy in 1969 and used by the skydiving team through 1977. After a short stint in storage at Tucson, it was assigned to the Nebraska Civil Air Patrol. It was acquired by the museum in 1996 from a private owner and put on display. It is currently in the Presidential Aircraft Gallery.